Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bioengineering-tissue strategies to model mammalian implantation in vitro

doi: 10.3389/fbioe.2024.1430235

Figure Lengend Snippet: List of primers used for quantitative PCR analysis.

Article Snippet: ZO1 , Tight Junction Protein ZO-1 , Ss03373514_m1.

Techniques: Real-time Polymerase Chain Reaction

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bioengineering-tissue strategies to model mammalian implantation in vitro

doi: 10.3389/fbioe.2024.1430235

Figure Lengend Snippet: Generation of the porcine 3D endometrial model and its characterization. (A) Schematic representation of the 3D endometrial model assembling, from uterine biopsy to endometrial epithelial cell and stromal fibroblast isolation and culture onto 2D standard plastic dishes (scale bar 100 μm). (B) Hematoxylin and Eosin staining of the 3D endometrial model obtained by co-culturing epithelial cells and stromal fibroblasts onto highly porous polystyrene scaffolds (scale bars 100 and 50 μm). (C) Transcription levels of VIM, COL3A1, KRT18, ZO1, CDH1 and EPCAM genes in endometrial stromal cells cultured on 2D culture systems (blue bars), endometrial epithelial cells cultured on 2D culture systems (pink bar), 3D endometrial models (yellow bars) and in vivo endometrial tissue, as a positive control (green bars). Gene expression is presented with the highest level set to 1 and all others relative to this. Data are expressed as the mean ± the standard error of the mean (SEM). a,b Different superscripts indicate p < 0.05. ND: not detected. (D) Immunofluorescent staining of 3D endometrial models for VIM (red) and ZO1 (green). Nuclei are counterstained with DAPI (blue) (scale bars 100 and 20 μm).

Article Snippet: ZO1 , Tight Junction Protein ZO-1 , Ss03373514_m1.

Techniques: Isolation, Staining, Cell Culture, In Vivo, Positive Control, Gene Expression

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Bioengineering-tissue strategies to model mammalian implantation in vitro

doi: 10.3389/fbioe.2024.1430235

Figure Lengend Snippet: Representative image of a TR spheroid attached to the 3D endometrial model. (A) Hematoxylin and Eosin staining of a TR spheroid adhering to the 3D endometrial culture system after a 24-h co-culture. No space between the spheroid and the epithelial compartment was visible (scale bar 50 μm). (B) Immunohistochemical co-staining for the mature TR marker GATA3 (red) and ZO1 (green) of a TR spheroid adhering to the 3D endometrial culture system. Nuclei were counterstained with DAPI (blue) (scale bars 100 μm).

Article Snippet: ZO1 , Tight Junction Protein ZO-1 , Ss03373514_m1.

Techniques: Staining, Co-Culture Assay, Immunohistochemical staining, Marker